Abstract: In Additive White Gaussian Noise (AWGN) channel, we introduce a method for synchronization of symbol timing and frequency for two-dimensional spread spectrum system without prior knowledge of spread spectrum matrix. Based on the periodical characteristic of the two-dimensional spread spectrum matrix, the method estimates the frequency offset, and then synchronizes the symbol timing by moving the position of data-storage window to calculate the maximum eigenvalue of correlation matrix. Computer simulations demonstrate the efficiency of this method.