Abstract: Multichannel sampling upon the frequency dimension is an important method to improve the overall sampling rate, where each channel needs to design an independent analog sampling filter and a digital reconfiguration filter to achieve the separation and undistorted recovery of the signal, resulting in high complexity of the structure and algorithm. In this paper, a method of frequency-interleaved sampling and reconstruction based on orthogonal frequency conversion is proposed. The sampling process divides the input signal into several sub-channels, where the radio frequency/intermediate frequency (RF/IF) channels are moved to the baseband by orthogonal mixing and sampling filter, and the quantization conversion is executed. The reconstruction process adopts digital up-conversion to move each channel back to its original carrier and obtain the output after frequency synthesis. The proposed method only needs one analog sampling filter and one digital reconstruction filter based on perfect reconstruction, which is more convenient for realization. Finally, the validity of the frequency-interleaved sampling and reconstruction method based on orthogonal frequency conversion is illustrated.