Abstract: Bacterial non-coding RNA (ncRNA) is a class of gene expression regulators in bacteria in recent years. Similar to the concept of essential genes, a part of ncRNA is indispensable for the survival of organisms, which is called essential ncRNA. Therefore, the bacterial essential ncRNAs could be used as potential targets for drug development in order to reduce drug resistance in pathogenic bacteria. The essential ncRNA has become one of the important objects of minimal genome research. At present, the essential ncRNAs of more than a dozen bacteria have been systematically identified by experimental method. However, there is no specialized essential ncRNAs database. And hence, the research on essential ncRNAs is far behind the needs of scientific research and drug design. In consequence, in this study, a special database of bacterial essential ncRNA (DBEncRNA) is proposed and constructed to help researchers develop the efficient recognition methods for essential ncRNA in silico, for further studies including antimicrobial target discovery and minimal genome research. The DBEncRNA database can be accessed by http://yeyn.group:86/ freely.