Abstract: Pilot-symbol-assisted channel estimation techniques of 2-D transform-domain OFDM system. The right-bottom zero-padding and middle zero-padding of pilot-position channel frequency response (CFR) matrix are utilized to gain the whole channel's CFR. To decrease the interpolation error of aforementioned zero-padding methods, a novel method is proposed which uses different number zeros and different padding mode based on pilot quantity along frequency and time direction. Lastly, by Simulink® baseband model, the proposed method is verified and compared with other 2-D interpolations. Results show that it can improve the channel estimation accuracy and decrease system bit error rate (BER) observably.