Abstract: Visual perceptual learning with aberration corrected by adaptive optics visual stimulator system (AOVS) is applied to human eyes. Four subjects with anisometropia and four subjects with strabismus are trained by this way. Statistical results show that based on the same method, the contrast sensitivity function (CSF) of four subjects with anisometropia are improved significantly than that of four subjects with strabismus. The comparison of visual acuity (VA) between two groups is performed before and after the training. Experiment results prove that the proposed method is effective for subjects with anisometropia but not effective for subjects with strabismus.