Abstract: Iris localization is the most critical step of an iris identification system. Since it determines the accuracy and efficiency of the system. To improve its accuracy and efficiency, we proposed a new iris localization algorithm based on Multi-resolution analysis to detect the edge points of iris at one appropriate resolution and set ellipses to these edge points. The most important merit of this algorithm is that it is seldom affected by lash occlusion problem and can localize the off-angle iris image.