Abstract: The kick-out pulse for calibration of sampling oscilloscopes is analyzed by Nose-to-Nose (NTN) method. The mathematic expressions of kick-out pulse parameter are deduced according to diode model of time-varying conductance. The factors influencing the kick-out pulse parameter are discussed. The emulational results show that the weaker the capacity of charging capacitance is, the wider the kick-out pulse spectrum bandwidth is. The asymmetry of diode inductance parameter can widen spectrum bandwidth, but increase overshoot. For the leak of strobe pulse, there exists groove in the kick-out pulse spectral response. Finally, the paper brings forward the requirements of the frequency band and the application condition of the NTN method.