Abstract: Inhibitors beta A (INHBA), as a member of transforming growth factor-β (TGF-β) superfamily, have pro-cancer or anti-cancer effects in different tumors. This study aims to investigate the expression and clinical significance of INHBA in colorectal cancer through bioinformatics databases. The results showed that INHBA expression was significantly upregulated in colorectal cancer, and its high expression was positively correlated with clinical stages, T stages and N stages of colorectal cancer. The methylation level of INHBA promoter was significantly reduced in both colon and rectal cancer. Survival analysis showed that colorectal cancer patients with high INHBA expression had poor prognosis. Moreover, INHBA expression was positively correlated with the infiltration levels of most immune cells in the tumor microenvironment, especially macrophages. INHBA high expression was also closely related to the high infiltration levels of cancer-associated fibroblasts (CAFs) and vascular endothelial cells (VECs). Taken together, INHBA is highly expressed in colorectal cancer tissues and involved in the development of colorectal cancer, which is expected to be a potential marker for prognosis and treatment.