Abstract: Since the three dimensional (3D) integrated circuit (IC) can offer shorter interconnection wire for network on chip (NoC), 3D NoC becomes an emerging research area in recent years. This paper proposes a new architecture based on De Bruijn graph for 3D NoC. It makes use of advantages of De Bruijn graph, such as small diameter, simple routing, and high reliability to design a hybrid layering architecture combined by horizontal planer network and virtual horizontal planer network. It also adopts fault-tolerant routing algorithm. This paper compares the performance of the proposed architecture with traditional 3D NoC architectures like 3D Mesh and XNoTs under uniform traffic and hotspot traffic. The simulation results prove that the De Bruijn graph based architecture can achieve smaller network latency than 3D Mesh based architectures with good scalability.