Abstract: In order to alleviate traffic congestion on freeways, national highways and provincial highways are used to achieve traffic diversion, and a targeted route guidance strategy is implemented. A hybrid routing model for regional multi-layer transportation network is established, which considers the congested driver sources. Firstly, a regional multi-layer transportation network composed of Hunan freeway, national highway and provincial highway is constructed, and the link weight is calibrated. Next, the congested driver sources are located, a hybrid routing model is established by considering the congested driver sources, and the targeted routing guidance is implemented for vehicles from the congested driver sources, so as to reduce the impact on travelers as much as possible while ensuring the mitigation effect of traffic congestion. Results show that the proposed route guidance model can effectively achieve traffic diversion and reduce the average travel time of vehicles in the multi-layer transportation network. In practical applications, the route guidance information can be only sent to a few drivers to reduce the difficulty of model implementation. The acceptance rate of drivers to the suggested routes can be analyzed to develop appropriate plans for disseminating route guidance information.